| Conference | Boys | Girls |
| Akron City Series | Firestone | Firestone |
| All-American Conference | Boardman | Boardman |
| Blanchard Valley Conference | Elmwood | Elmwood |
| Buckeye Border Conference | Pettisville | Holgate |
| Central Buckeye Conference - KT | Jonathan Alder | Jonathan Alder |
| Central Buckeye Conference - MR | North Union | North Union |
| Central Buckeye League | Bexley | Grandview Heights |
| Central Catholic League | Bishop Watterson | Bishop Watterson |
| Chagrin Valley Conference - Chagrin | Orange | Geneva |
| Chagrin Valley Conference - Lake | Chardon | Chardon |
| Chagrin Valley Conference - Metro | Independence | Beachwood |
| Chagrin Valley Conference - Valley | Chagrin Falls | Kirtland |
| Cincinnati Hills League | Madeira | Indian Hill |
| Cincinnati Metro Athletic Conference | Hughes | SCPA |
| Cleveland Senate Athletic League | Rhodes | John Hay |
| Cleveland West Conference | Rocky River | Bay |
| Columbus City League | Whetstone | Whetstone |
| Dayton City League | Meadowdale | |
| Eastern Buckeye Conference | Salem | Minerva |
| Eastern Cincinnati Conference | Milford | Milford |
| Eastern Ohio Athletic Conference | Columbiana | Columbiana |
| Federal League | Massillon Jackson | Massillon Jackson |
| Firelands Conference | Ashland Crestview | Norwalk St. Paul |
| Frontier Athletic Conference | Jackson | Chillicothe |
| Greater Catholic League - Co-Ed | Dayton Carroll | Bishop Fenwick |
| Greater Catholic League - South | St. Xavier | Cin. St. Ursula |
| Greater Cleveland Conference | Mentor | Shaker Heights |
| Greater Miami Conference | Mason | Oak Hills |
| Greater Western Ohio Conference | Springboro | Springboro |
| Green Meadows Conference | Tinora | Tinora |
| Inter-Valley Conference | Tusky Valley | Tusky Valley |
| Knox Morrow Athletic Conference | Mount Gilead | Mount Gilead |
| Licking County League - Buckeye | Granville | Granville |
| Licking County League - Cardinal | Utica | Johnstown-Monroe |
| Lorain County League | Keystone | Firelands |
| Mahoning Valley Athletic Conference | Garrettsville Garfield | McDonald |
| Metro Athletic Conference | Woodridge | Tallmadge |
| Miami Valley Conference | CHCA | Summit Country Day |
| Mid-Buckeye Conference | Kidron Central Christian | Crestline |
| Mid-Ohio Athletic Conference | Marion Harding | Shelby |
| Mid-Ohio Christian Athletic League | Tree of Life Christian | Westerville Genoa Christian |
| Mid-State League - Buckeye | Fairfield Union | Fairfield Union |
| Mid-State League - Cardinal | Fisher Catholic | Fisher Catholic |
| Midwest Athletic Conference | Versailles | Minster |
| Muskingum Valley League | Sheridan | Sheridan |
| North Coast Conference | CVCA | CVCA |
| Northeast 8 Athletic Conference | Hubbard | Poland Seminary |
| Northeastern Athletic Conference | Maplewood | Maplewood |
| Northern 10 Athletic Conference | Upper Sandusky | Carey |
| Northern Buckeye Conference | Eastwood | Maumee |
| Northern Lakes League - Buckeye | Perrysburg | Perrysburg |
| Northern Lakes League - Cardinal | Bowling Green | Fremont Ross |
| Northern Ohio Conference | Tiffin Columbian | Huron |
| Northwest Central Conference | Cory-Rawson | North Baltimore |
| Northwest Conference | Convoy Crestview | Lincolnview |
| Northwest Ohio Athletic League | Bryan | Liberty Center |
| Ohio Capital Conference - Buckeye | Canal Winchester | Canal Winchester |
| Ohio Capital Conference - Capital | Westerville North | Big Walnut |
| Ohio Capital Conference - Cardinal | Olentangy Berlin | Dublin Jerome |
| Ohio Capital Conference - Central | Olentangy Liberty | Upper Arlington |
| Ohio Capital Conference - Ohio | Gahanna Lincoln | Gahanna Lincoln |
| Ohio Cardinal Conference | Lexington | Lexington |
| Ohio Heritage Conference | West Liberty-Salem | West Liberty-Salem |
| Ohio Valley Athletic Conference 1-2A | Trinity Christian | Shadyside |
| Ohio Valley Athletic Conference 3A | Shendandoah | Bellaire |
| Ohio Valley Athletic Conference 4A | Martins Ferry | Richmond Edison |
| Ohio Valley Athletic Conference 5A | Morgantown | Morgantown |
| Ohio Valley Conference | Gallia Academy | Fairland |
| Principals Athletic Conference | Fairless | Canal Fulton Northwest |
| Sandusky Bay Conference | Margaretta | Ottawa Hills |
| Scioto Valley Conference | Unioto | Unioto |
| Shelby County Athletic League | Russia | Fort Loramie |
| Southern Buckeye Athletic/Academic Conference - A | Western Brown | Western Brown |
| Southern Buckeye Athletic/Academic Conference - N | Williamsburg | Georgetown |
| Southern Ohio Conference - I | Symmes Valley | Portsmouth Clay |
| Southern Ohio Conference - II | South Webster | Waverly |
| Southwestern Buckeye League - Buckeye | Oakwood | Oakwood |
| Southwestern Buckeye League - Southwestern | Bellbrook | Bellbrook |
| Southwestern Conference | Avon | Avon |
| Suburban League - American | Kenston | Aurora |
| Suburban League - National | Solon | Stow-Munroe Falls |
| Three Rivers Conference | Milton-Union | Milton-Union |
| Toledo Area Athletic Conference | Maumee VCD | |
| Toledo City League | Waite | Rogers |
| Tri-Valley Conference - Hocking | Racine Southern | Belpre |
| Tri-Valley Conference - Ohio | Vinton County | Athens |
| Wayne County Athletic League | Norwayne | Rittman |
| Western Buckeye League | Van Wert | Ottawa-Glandorf |
| Western Ohio Athletic Conference | Tri-Village | Arcanum |